منابع مشابه
Isolation of lamellar bodies from neonatal mouse epidermis by selective sequential filtration.
Isolation of epidermal lamellar bodies has presented a challenge because pressures required to homogenize keratinocytes can destroy these organelles and because the lamellar body readily releases its contents during prolonged isolation procedures. In an attempt to isolate lamellar bodies, sheets of intact stratum corneum and stratum granulosum were obtained from neonatal mice with highly purifi...
متن کاملSurface-expressed lamellar body membrane is recycled to lamellar bodies.
Monoclonal antibody (MAb) 3C9, an antibody generated to the lamellar body of rat lung type II pneumocytes, specifically labels the luminal face of the lamellar body membrane. To follow the retrieval of lamellar body membrane from the cell surface in these cells, MAb 3C9 was instilled into rat lungs. In vivo, it was endocytosed by type II cells but not by other lung cells. In type II cells that ...
متن کاملHydrolases of pulmonary lysosomes and lamellar bodies.
Lamellar bodies and lysosomes from the lungs of rabbits were examined for the presence of 24 hydrolytic enzymes. All hydrolases detectable in lysosomes were also found in lamellar bodies. Hydrolase activities in lamellar bodies were not due to contamination with lysosomes as shown by isopycnic centrifugation of the subcellular organelles on continuous density sucrose gradients. Lysosomal prepar...
متن کاملPerinuclear sudanophil bodies in mammalian epidermis.
1. In the epidermis of laboratory mammals there are perinuclear lipid spherules which are demonstrated clearly with sudan black. The centre of the larger of these droplets is occupied by a sudanophobe core of varying dimensions. 2. The distribution of the bodies corresponds to the osmiophil elements in comparable tissues, and the structural pattern is similar to that of the Golgi elements in ot...
متن کاملLamellar bodies of human epidermis: proteomics characterization by high throughput mass spectrometry and possible involvement of CLIP-170 in their trafficking/secretion.
Lamellar bodies (LBs) are tubulovesicular secretory organelles of epithelial cells related to lysosomes. In the epidermis, they play a crucial role in permeability barrier homeostasis, secreting their contents, lipids, a variety of hydrolases, protease inhibitors, and antimicrobial peptides, in the upper keratinocyte layers. The identification of proteins transported in epidermal LBs is still f...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2008
ISSN: 1535-9476
DOI: 10.1074/mcp.m700334-mcp200